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Immunology and Infection
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JoVE Journal Immunology and Infection
Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages
 

Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages

Article DOI: 10.3791/50625-v • 13:48 min • December 10th, 2013
December 10th, 2013 •
1, 1, *1, *1,2, 1, 1
1Laboratory of Fungal Pathogenesis, Centre for DNA Fingerprinting and Diagnostics, Andhra Pradesh, India, 2Current location: VIB Department for Molecular Biomedical Research, UGent, Fiers-Schell-Van Montagu Building, Technologiepark 927, B-9052 Ghent (Zwijnaarde), Belgium
* These authors contributed equally

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The current article outlines a protocol to establish an in vitro cell culture model system to study the interaction of a facultative intracellular human fungal pathogen Candida glabrata with human macrophages which will be a useful tool to advance our knowledge of fungal virulence mechanisms.

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In Vitro System Candida Glabrata THP-1 Macrophages Cell Culture Model Microbial Pathogen Infection Virulence Strategies Opportunistic Yeast Pathogen Host Phagocytic Cells Mutant Screens Viability Analysis Immune Cell Circuitry Fungal Pathogen Evasion
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