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Neuroscience
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激光捕获显微切割的丰富种群的创伤性脑损伤后神经元或单个神经元的基因表达分析
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury
Please note that all translations are automatically generated.
Click here for the English version.
激光捕获显微切割的丰富种群的创伤性脑损伤后神经元或单个神经元的基因表达分析
DOI:
10.3791/50308-v
•
13:32 min
•
April 10, 2013
•
Deborah R. Boone
,
Stacy L. Sell
,
Helen Lee Hellmich
1
Department of Anesthesiology
,
University of Texas Medical Branch
Chapters
00:05
Title
02:00
Sectioning and Staining of Rat Brain for LCM
04:28
LCM of Swaths of Enriched Populations of Hippocampal Neurons
08:41
LCM of Single FJ+ Neurons in the Hippocampus
10:42
Results: Laser Capture Microdissection of Individual and Swaths of Hippocampal Neurons
12:51
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了如何使用激光捕获显微切割(LCM),以获得丰富的海马神经元群体或单个神经元,随后采用实时定量PCR和/或整个基因组微阵列基因表达分析的损伤大鼠的大脑冰冻切片。
Tags
Laser Capture Microdissection
LCM
Gene Expression Analysis
Traumatic Brain Injury
TBI
Hippocampus
Neurons
Neurodegeneration
Real-time PCR
Microarray
Suprachiasmatic Nuclei
Circadian Rhythms
Cell Death
Cell Survival
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