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12:02 min
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May 24, 2010
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In this video-article we present a method for isolating single or multiple Drosophila da neurons from third instar larvae using the infrared capture (IR) class of Laser Capture Microdissection (LCM). RNA obtained from the isolated neurons can be readily used for downstream applications including qRT-PCR or microarray analyses.
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Laser Capture MicrodissectionDrosophila Peripheral NeuronsDendritic ArborizationDa NeuronsMolecular MechanismsClass-specific Dendrite MorphogenesisRNA Isolation TechniquesMagnetic Bead-based Cell PurificationFluorescent Activated Cell Sorting (FACS)RNA Binding Protein-based StrategiesLaser Capture Microdissection (LCM)Spatial PrecisionQRT-PCRMicroarray Expression ProfilingThird Instar Larval Stage
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Iyer, E. P. R., Cox, D. N. Laser Capture Microdissection of Drosophila Peripheral Neurons. J. Vis. Exp. (39), e2016, doi:10.3791/2016 (2010).
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