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Journal / Neuroscience / Production of RNA for Transcriptomic Analysis…
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Neuroscience

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JoVE Journal Neuroscience
Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection
 

Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection

Article DOI: 10.3791/51168-v • 11:46 min • January 13th, 2014
January 13th, 2014 •
1,2, 1, 1,2, 1,2
1Department of Genetics, Yale School of Medicine, 2Howard Hughes Medical Institute

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High-quality total RNA has been prepared from cell bodies of mouse spinal cord motor neurons by laser capture microdissection after staining spinal cord sections with Azure B in 70% ethanol. Sufficient RNA (~40-60 ng) is recovered from 3,000-4,000 motor neurons to allow downstream RNA analysis by RNA-seq and qRT-PCR.

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RNA Production Transcriptomic Analysis Mouse Spinal Cord Motor Neuron Cell Bodies Laser Capture Microdissection High-quality RNA Transcriptional Differences Neurologic Diseases Neurodegenerative Diseases Pharmacologic Treatments LMD Protocol RNA Damage Endogenous RNases Exogenous RNases Azure B Staining Motor Neurons Identification Guanidine Thiocyanate Lysis Buffer RNA Integrity Downstream Analysis RNA-seq QRT-PCR
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