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Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

DOI:
10.3791/50308-v

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13:32 min

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April 10, 2013

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, ,
1Department of Anesthesiology,University of Texas Medical Branch

Capitoli

  • 00:05Titolo
  • 02:00Sectioning and Staining of Rat Brain for LCM
  • 04:28LCM of Swaths of Enriched Populations of Hippocampal Neurons
  • 08:41LCM of Single FJ+ Neurons in the Hippocampus
  • 10:42Results: Laser Capture Microdissection of Individual and Swaths of Hippocampal Neurons
  • 12:51Conclusion

Summary

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We describe how to use laser capture microdissection (LCM) to obtain enriched populations of hippocampal neurons or single neurons from frozen sections of the injured rat brain for subsequent gene expression analysis using quantitative real time PCR and/or whole-genome microarrays.

Tags

Laser Capture MicrodissectionLCMGene Expression AnalysisTraumatic Brain InjuryTBIHippocampusNeuronsNeurodegenerationReal-time PCRMicroarraySuprachiasmatic NucleiCircadian RhythmsCell DeathCell Survival

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