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Journal / Neuroscience / Laser Capture Microdissection of Enriched…
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Neuroscience

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JoVE Journal Neuroscience
Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury
 

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Article DOI: 10.3791/50308-v • 13:32 min • April 10th, 2013
April 10th, 2013 •
1, 1, 1
1Department of Anesthesiology, University of Texas Medical Branch

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요약
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We describe how to use laser capture microdissection (LCM) to obtain enriched populations of hippocampal neurons or single neurons from frozen sections of the injured rat brain for subsequent gene expression analysis using quantitative real time PCR and/or whole-genome microarrays.

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Laser Capture Microdissection Enriched Populations Of Neurons Single Neurons Gene Expression Analysis Traumatic Brain Injury Cognitive Disability Neurodegeneration Hippocampus Learning Memory Executive Function Specific Neuronal Populations Subregions Of The Hippocampus Technique Emmert-Buck Advances Heterogeneous Tissues Mammalian Brain Functional Cell Types Rat Model Of Traumatic Brain Injury Molecular Mechanisms Pathogenesis Of TBI Fluid-Percussion TBI Cryostat OCT Embedding Circadian Rhythms Suprachiasmatic Nuclei Master Clock
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