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Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue
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Neuroscience
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JoVE Journal Neuroscience
Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue

Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue

English

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28,476 Views

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08:37 min

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August 27, 2016

DOI:

10.3791/54358-v

DOI:
10.3791/54358-v

•

08:37 min
August 27, 2016

•

35 Views
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1Behavioral Neuroscience Research Branch, Intramural Research Program,NIDA, NIH, DHHS, 2Division of Rheumatology, School of Medicine,Johns Hopkins University

Summary

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Here we present a Fluorescence Activated Cell Sorting (FACS) protocol to study molecular alterations in Fos-expressing neuronal ensembles from both fresh and frozen brain tissue. The use of frozen tissue allows FACS isolation of many brain areas over multiple sessions to maximize the use of valuable animal subjects.

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Rubio, F. J., Li, X., Liu, Q., Cimbro, R., Hope, B. T. Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue. J. Vis. Exp. (114), e54358, doi:10.3791/54358 (2016).

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