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Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders
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Neuroscience
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JoVE Journal Neuroscience
Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders

Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders

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9,621 Views

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08:33 min

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December 04, 2014

DOI:

10.3791/51853-v

DOI:
10.3791/51853-v

•

08:33 min
December 04, 2014

•

9 Views
, , , , , , , , ,
1Department of Psychiatry,Johns Hopkins University, 2Department of Psychiatry and Behavioral Sciences,Howard University, 3Department of Otolaryngology-Head and Neck Surgery,Johns Hopkins University, 4Department of Psychiatry,Sheppard Pratt Hospital, 5Department of Psychiatry,Indiana University

Summary

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In this study, a novel platform to investigate intraneuronal molecular signatures of treatment response in bipolar disorder (BD) was developed and validated. Olfactory epithelium from BD patients was obtained through nasal biopsies. Then laser-capture microdissection was combined with Real Time RT PCR to investigate the molecular signature of lithium response in BD.

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Olfactory NeuronsNasal BiopsyLaser-capture MicrodissectionBipolar DisorderLithium CarbonateWnt SignalingNeuropsychiatric DisordersOlfactory EpitheliumTreatment Response

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Narayan, S., McLean, C., Sawa, A., Lin, S. Y., Rai, N., Hipolito, M. S., Cascella, N., Nurnberger, Jr., J. J., Ishizuka, K., Nwulia, E. A. Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders. J. Vis. Exp. (94), e51853, doi:10.3791/51853 (2014).

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