Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

St&#233;phanie Beaucourt; Antonio V. Border&#237;a; Lark L. Coffey; Nina F. Gn&#228;dig; Marta Sanz-Ramos; Yasnee Beeharry; Marco Vignuzzi

doi:10.3791/2953

Journal

/

Immunology and Infection

/

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

29,273 Views

•

18:10 min

•

June 16, 2011

DOI:

10.3791/2953-v

DOI:

10.3791/2953-v

•

18:10 min

June 16, 2011

•

4 Views

Stéphanie Beaucourt¹, Antonio V. Bordería¹, Lark L. Coffey¹, Nina F. Gnädig¹, Marta Sanz-Ramos¹, Yasnee Beeharry¹, Marco Vignuzzi¹

¹Viral Populations and Pathogenesis lab and CNRS 3015,Institut Pasteur

Summary

The present article describes the steps required to isolate and characterize RNA polymerase fidelity variants of RNA viruses and how to use mutation frequency data to confirm fidelity changes in tissue culture.

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RNA Viruses Fidelity Variants Virus Mutation Frequency RNA Dependent RNA Polymerases Extreme Population Diversity Virus Adaptation Virus Evolution Intrinsic Error Rates Viral Polymerase Incorporation Fidelity Mutation Frequencies Virological Techniques Sequencing Techniques Statistical Significance Polymerase Structure And Function Virus Adaptation Mechanisms

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Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

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