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Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

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20,986 Views

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12:20 min

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December 29, 2015

DOI:

10.3791/53164-v

DOI:
10.3791/53164-v

•

12:20 min
December 29, 2015

•

4 Views
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1Department of Animal, Dairy, and Veterinary Sciences, Utah Science Technology and Research, College of Agriculture and Applied Sciences,Utah State University

Summary

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Here, a protocol is presented for creating an infectious bacterial artificial chromosome containing a full-length cDNA of the positive-strand genomic RNA of Japanese encephalitis virus. This protocol can be used to construct a functional cDNA of other positive-strand RNA viruses, making it a powerful genomic tool for studying virus biology.

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Bacterial Artificial ChromosomesReverse GeneticsPositive-strand RNA VirusesCDNAInfectious VirusViral Genomic RNAViral ReplicationViral PathogenesisViral VectorsJapanese Encephalitis VirusGenome StabilityFull-length Infectious CDNARNA SynthesisIn Vitro Transcription

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Yun, S., Song, B., Kim, J., Lee, Y. Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses. J. Vis. Exp. (106), e53164, doi:10.3791/53164 (2015).

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