Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

St&#233;phanie Beaucourt; Antonio V. Border&#237;a; Lark L. Coffey; Nina F. Gn&#228;dig; Marta Sanz-Ramos; Yasnee Beeharry; Marco Vignuzzi

doi:10.3791/2953

Journal

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Immunology and Infection

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Isolatie van Fidelity Varianten van RNA-virussen en Karakterisering van mutatie van de virussen Frequency

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

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Isolatie van Fidelity Varianten van RNA-virussen en Karakterisering van mutatie van de virussen Frequency

29,275 Views

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18:10 min

•

June 16, 2011

DOI:

10.3791/2953-v

DOI:

10.3791/2953-v

•

18:10 min

June 16, 2011

•

4 Views

Stéphanie Beaucourt¹, Antonio V. Bordería¹, Lark L. Coffey¹, Nina F. Gnädig¹, Marta Sanz-Ramos¹, Yasnee Beeharry¹, Marco Vignuzzi¹

¹Viral Populations and Pathogenesis lab and CNRS 3015,Institut Pasteur

Summary

In dit artikel beschrijft de stappen die nodig zijn om te isoleren en te karakteriseren RNA polymerase trouw varianten van RNA-virussen en hoe je mutatiefrequentie gegevens gebruiken om trouw veranderingen in weefselkweek te bevestigen.

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Isolatie van Fidelity Varianten van RNA-virussen en Karakterisering van mutatie van de virussen Frequency

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Summary

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