Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

St&#233;phanie Beaucourt; Antonio V. Border&#237;a; Lark L. Coffey; Nina F. Gn&#228;dig; Marta Sanz-Ramos; Yasnee Beeharry; Marco Vignuzzi

doi:10.3791/2953

Journal

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Immunology and Infection

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Isolamento di varianti di virus RNA Fidelity e caratterizzazione di frequenza Virus Mutation

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

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Isolamento di varianti di virus RNA Fidelity e caratterizzazione di frequenza Virus Mutation

29,213 Views

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18:10 min

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June 16, 2011

DOI:

10.3791/2953-v

DOI:

10.3791/2953-v

•

18:10 min

June 16, 2011

•

4 Views

Stéphanie Beaucourt¹, Antonio V. Bordería¹, Lark L. Coffey¹, Nina F. Gnädig¹, Marta Sanz-Ramos¹, Yasnee Beeharry¹, Marco Vignuzzi¹

¹Viral Populations and Pathogenesis lab and CNRS 3015,Institut Pasteur

Summary

Il presente articolo descrive i passaggi necessari per isolare e caratterizzare l'RNA polimerasi varianti fedeltà dei virus a RNA e come utilizzare i dati di frequenza di mutazione per confermare le modifiche fedeltà in coltura.

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Isolamento di varianti di virus RNA Fidelity e caratterizzazione di frequenza Virus Mutation

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Summary

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