/
/
Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
This content is Free Access.
Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
29,275 Views
•
18:10 min
•
June 16, 2011
DOI:
Summary
Automatically generated
The present article describes the steps required to isolate and characterize RNA polymerase fidelity variants of RNA viruses and how to use mutation frequency data to confirm fidelity changes in tissue culture.
Explore More Videos
RNA VirusesFidelity VariantsVirus Mutation FrequencyRNA Dependent RNA PolymerasesExtreme Population DiversityVirus AdaptationVirus EvolutionIntrinsic Error RatesViral PolymeraseIncorporation FidelityMutation FrequenciesVirological TechniquesSequencing TechniquesStatistical SignificancePolymerase Structure And FunctionVirus Adaptation Mechanisms
Read Article
Cite this Article
Beaucourt, S., Bordería, A. V., Coffey, L. L., Gnädig, N. F., Sanz-Ramos, M., Beeharry, Y., Vignuzzi, M. Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency. J. Vis. Exp. (52), e2953, doi:10.3791/2953 (2011).
Copy