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Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

DOI:
10.3791/2953-v

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18:10 min

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June 16, 2011

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, , , , , ,
1Viral Populations and Pathogenesis lab and CNRS 3015,Institut Pasteur

Chapters

  • 00:05Title
  • 01:54Determining Mutagen Toxicity for Cells
  • 03:01Determining Mutagen Concentrations that Optimally Reduce Virus Titers
  • 04:56Isolation and Identification of Mutagen Resistant Variants
  • 06:41Confirming the Resistance Phenotype
  • 08:32Checking Replication Rates and Measuring Mutation Frequencies
  • 10:40Sequence Analysis
  • 12:34Representative Results
  • 17:27Conclusion

Summary

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The present article describes the steps required to isolate and characterize RNA polymerase fidelity variants of RNA viruses and how to use mutation frequency data to confirm fidelity changes in tissue culture.

Tags

RNA VirusesFidelity VariantsVirus Mutation FrequencyRNA Dependent RNA PolymerasesExtreme Population DiversityVirus AdaptationVirus EvolutionIntrinsic Error RatesViral PolymeraseIncorporation FidelityMutation FrequenciesVirological TechniquesSequencing TechniquesStatistical SignificancePolymerase Structure And FunctionVirus Adaptation Mechanisms

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