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Immunology and Infection

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HLA - IG基于人工抗原提呈细胞可高效体外扩展人类的CTL

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL

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HLA - IG基于人工抗原提呈细胞可高效体外扩展人类的CTL

DOI:

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07:18 min

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April 11, 2011

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Yen-Ling Chiu², Jonathan P. Schneck⁴, Mathias Oelke⁴

¹Immunology Graduate Program,Johns Hopkins University, ²Department of Internal Medicine,Far-Eastern Memorial Hospital, ³Department of Pathology,Johns Hopkins University, ⁴Institute of Cell Engineering,Johns Hopkins University

Chapters

00:05Title
01:39Making HLA-A2-Ig-based aAPC
02:47Quality Control of aAPC and Peptide Loading and Storage
03:43Human CTL Isolation
05:22In Vitro aAPC-based Culture System
06:25Expanded CTL Are Polyfunctional – Not Exhausted – after Prolonged Cell Culture and Proliferation
06:49Conclusion

Summary

Automatic Translation

描述一个新的DC抗原特异性T细胞的诱导和扩张的独立方法。基于HLA A2 - Ig的人工抗原提呈细胞（AAPC）加载与HLA - A2限制性肽能够有效地扩大多样化的抗原特异性CTL的。该技术拥有CTL为基础的过继免疫治疗的巨大潜力。

Tags

HLA-Ig Artificial Antigen Presenting Cells Ex Vivo Expansion Human CTL Effector Function Immune Microenvironment Autologous Antigen Presenting Cells Tolerize Anergize Adoptive Immunotherapy Cancer Virus Infections CMV-specific CTL Immunocompromised Allogeneic Bone Marrow Transplant Patients Anti-CMV Immunity Melanoma Cost And Technical Limitations Autologous DC Leukocytes

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