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Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
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Immunology and Infection
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JoVE Journal Immunology and Infection
Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

English

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14,279 Views

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10:31 min

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April 08, 2016

DOI:

10.3791/52564-v

DOI:
10.3791/52564-v

•

10:31 min
April 08, 2016

•

9 Views
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1Laboratory of Medical Immunology, Department of Laboratory Medicine,Radboud University Medical Centre, 2Department of Dermatology,Radboud University Medical Centre

Summary

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We describe a protocol to efficiently isolate skin resident T cells from human skin biopsies. This protocol yields sufficient numbers of viable human skin resident lymphocytes for flow cytometric analysis and ex vivo culture.

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Lymphocyte IsolationSkin BiopsyT CellsFlow CytometryEx Vivo Cell CultureSingle Cell Suspension

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Cite this Article

He, X., de Oliveira, V. L., Keijsers, R., Joosten, I., Koenen, H. J. Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture. J. Vis. Exp. (110), e52564, doi:10.3791/52564 (2016).

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