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HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL

HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL

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07:18 min

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April 11, 2011

DOI:

10.3791/2801-v

DOI:
10.3791/2801-v

•

07:18 min
April 11, 2011

•

3 Views
, ,
1Immunology Graduate Program,Johns Hopkins University, 2Department of Internal Medicine,Far-Eastern Memorial Hospital, 3Department of Pathology,Johns Hopkins University, 4Institute of Cell Engineering,Johns Hopkins University

Summary

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A new DC independent method for induction and expansion of antigen-specific T cells is described. HLA A2-Ig based artificial Antigen Presenting Cells (aAPC) are loaded with HLA-A2 restricted peptides to efficiently expand CTL of diverse antigen specificity. This technology holds great potential for CTL-based adoptive immunotherapy.

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Cite this Article

Chiu, Y., Schneck, J. P., Oelke, M. HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL. J. Vis. Exp. (50), e2801, doi:10.3791/2801 (2011).

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