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Expansion of Human Peripheral Blood γδ T Cells using Zoledronate
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September 09, 2011
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Summary
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A method to expand γδ T cells from peripheral blood mononuclear cells (PBMC) is described. PBMC-derived γδ T cells are stimulated and expanded using zoledronate and interleukin-2 (IL-2). Large scale expansion of γδ T cells can be applied to autologous cellular immunotherapy of cancer.
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Kondo, M., Izumi, T., Fujieda, N., Kondo, A., Morishita, T., Matsushita, H., Kakimi, K. Expansion of Human Peripheral Blood γδ T Cells using Zoledronate. J. Vis. Exp. (55), e3182, doi:10.3791/3182 (2011).
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