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Isolation und Expansion der erwachsenen Maus Neural Stem Cells mit dem Neurosphäre Assay
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Chapters
- 00:00Title
- 00:41Introduction
- 01:04Animal Dissection
- 02:25Brain Dissection
- 04:31Tissue Preparation and Plating Cells
- 09:04Passaging Neurospheres
- 11:56Representative Results
- 12:55Conclusion
Dieses Video-Protokoll zeigt die Neurosphäre Assay-Methode zu generieren und zu erweitern neurale Stammzellen aus dem erwachsenen Maus periventrikulären Region und erbringt technische Erkenntnisse, um sicherzustellen, kann man reproduzierbare Neurosphäre Kulturen zu erreichen.
Tags
IsolationExpansionAdult MouseNeural Stem CellsNeurosphere AssayPutativeMurine BrainReynolds And WeissChemically Defined Serum-free Culture SystemGrowth Factor Responsive Precursor CellsEpidermal Growth FactorBasic Fibroblastic Growth FactorColoniesUndifferentiated CellsNeurospheresSubculturedPool Of Neural Stem CellsDifferentiateThree Major Cell TypesCNSNeuronsAstrocytesOligodendrocytesInvaluable ToolIn Vitro StudiesTherapeutic PurposesVideo DemonstrationPeriventricular RegionReproducible Neurosphere Cultures
