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Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay
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Neuroscience
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JoVE Journal Neuroscience
Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

English

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24,387 Views

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11:54 min

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January 11, 2011

DOI:

10.3791/2457-v

DOI:
10.3791/2457-v

•

11:54 min
January 11, 2011

•

22 Views
, , , ,
1Department of Anatomical Sciences,Shiraz University of Medical Sciences, Shiraz, Iran , 2Department of Neurosurgery,The University of Florida

Summary

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This video protocol demonstrates the application of the neurosphere assay for the isolation and expansion of neural stem cells from the ganglionic eminences of embryonic day 14-mouse brain.

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Azari, H., Sharififar, S., Rahman, M., Ansari, S., Reynolds, B. A. Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay. J. Vis. Exp. (47), e2457, doi:10.3791/2457 (2011).

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