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Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
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Neuroscience
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JoVE Journal Neuroscience
Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

English

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26,557 Views

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13:21 min

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November 20, 2010

DOI:

10.3791/2393-v

DOI:
10.3791/2393-v

•

13:21 min
November 20, 2010

•

5 Views
, , ,
1 Department of Anatomical Sciences,Shiraz University of Medical Sciences, Shiraz, Iran , 2Department of Neurosurgery,University of Florida

Summary

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This video protocol demonstrates the neurosphere assay method to generate and expand neural stem cells from the adult mouse periventricular region, and provides technical insights to ensure one can achieve reproducible neurosphere cultures.

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Cite this Article

Azari, H., Rahman, M., Sharififar, S., Reynolds, B. A. Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay. J. Vis. Exp. (45), e2393, doi:10.3791/2393 (2010).

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