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Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

26,557 Views

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13:21 min

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November 20, 2010

DOI:

10.3791/2393-v

DOI:
10.3791/2393-v

•

13:21 min
November 20, 2010

•

5 Views
, , ,
1 Department of Anatomical Sciences,Shiraz University of Medical Sciences, Shiraz, Iran , 2Department of Neurosurgery,University of Florida

Summary

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This video protocol demonstrates the neurosphere assay method to generate and expand neural stem cells from the adult mouse periventricular region, and provides technical insights to ensure one can achieve reproducible neurosphere cultures.

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Azari, H., Rahman, M., Sharififar, S., Reynolds, B. A. Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay. J. Vis. Exp. (45), e2393, doi:10.3791/2393 (2010).

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