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Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
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Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
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13:21 min
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November 20, 2010
DOI:
Summary
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This video protocol demonstrates the neurosphere assay method to generate and expand neural stem cells from the adult mouse periventricular region, and provides technical insights to ensure one can achieve reproducible neurosphere cultures.
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Azari, H., Rahman, M., Sharififar, S., Reynolds, B. A. Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay. J. Vis. Exp. (45), e2393, doi:10.3791/2393 (2010).
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