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Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay

Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay

English

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29,368 Views

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09:24 min

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October 30, 2011

DOI:

10.3791/3633-v

DOI:
10.3791/3633-v

•

09:24 min
October 30, 2011

•

9 Views
, , , , ,
1Department of Neurosurgery,University of Florida , 2Department of Anatomical Sciences,Shiraz University of Medical Sciences

Summary

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This video protocol demonstrates the isolation and expansion of stem like cells from surgically resected human glioblastoma mutliforme (GBM) tumor tissue using the neurosphere assay culture method.

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Azari, H., Millette, S., Ansari, S., Rahman, M., Deleyrolle, L. P., Reynolds, B. A. Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay. J. Vis. Exp. (56), e3633, doi:10.3791/3633 (2011).

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