/
/
Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
26,557 Views
•
13:21 min
•
November 20, 2010
DOI:
Summary
Automatically generated
This video protocol demonstrates the neurosphere assay method to generate and expand neural stem cells from the adult mouse periventricular region, and provides technical insights to ensure one can achieve reproducible neurosphere cultures.
Explore More Videos
IsolationExpansionAdult MouseNeural Stem CellsNeurosphere AssayPutativeMurine BrainReynolds And WeissChemically Defined Serum-free Culture SystemGrowth Factor Responsive Precursor CellsEpidermal Growth FactorBasic Fibroblastic Growth FactorColoniesUndifferentiated CellsNeurospheresSubculturedPool Of Neural Stem CellsDifferentiateThree Major Cell TypesCNSNeuronsAstrocytesOligodendrocytesInvaluable ToolIn Vitro StudiesTherapeutic PurposesVideo DemonstrationPeriventricular RegionReproducible Neurosphere Cultures
Read Article
Cite this Article
Azari, H., Rahman, M., Sharififar, S., Reynolds, B. A. Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay. J. Vis. Exp. (45), e2393, doi:10.3791/2393 (2010).
Copy