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Medición de la oxigenación sanguínea cerebral basada en el oxígeno-dependiente de enfriamiento de fosforescencia
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Neuroscience
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JoVE Journal Neuroscience
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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Medición de la oxigenación sanguínea cerebral basada en el oxígeno-dependiente de enfriamiento de fosforescencia

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14,526 Views

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08:58 min

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May 04, 2011

DOI:

10.3791/1694-v

DOI:
10.3791/1694-v

•

08:58 min
May 04, 2011

•

3 Views
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1Optics Division, Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology,Massachusetts General Hospital and Harvard Medical School, 2Department of Biochemistry and Biophysics,University of Pennsylvania, 3Neuroprotection Research Laboratory, Departments of Radiology and Neurology,Massachusetts General Hospital and Harvard Medical School, 4Departments of Neurosciences and Radiology,University of California

Summary

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Se presenta un procedimiento experimental para la medición de la presión parcial de oxígeno (pO2) en la vasculatura cerebral basada en el oxígeno-dependiente de extinción de la fosforescencia. Preparación de animales y los procedimientos de imagen se describe, tanto para el campo visual amplio CCD basado en imágenes de la pO2 de las ratas y 2-fotones de imágenes basado en la excitación de la pO2 de ratones.

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Sakadžić, S., Roussakis, E., Yaseen, M. A., Mandeville, E. T., Srinivasan, V. J., Arai, K., Ruvinskaya, S., Wu, W., Devor, A., Lo, E. H., Vinogradov, S. A., Boas, D. A. Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence. J. Vis. Exp. (51), e1694, doi:10.3791/1694 (2011).

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