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Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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08:58 min
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May 04, 2011
DOI:
1Optics Division, Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology,Massachusetts General Hospital and Harvard Medical School, 2Department of Biochemistry and Biophysics,University of Pennsylvania, 3Neuroprotection Research Laboratory, Departments of Radiology and Neurology,Massachusetts General Hospital and Harvard Medical School, 4Departments of Neurosciences and Radiology,University of California
Summary
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We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.
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Cerebral Blood Oxygenation MeasurementOxygen-dependent Quenching Of PhosphorescenceSpatiotemporal CharacteristicsNeuro-metabolic-vascular RelationshipPO2 Measurement ModalitiesLarger Fields Of ViewHigher Spatial ResolutionHigher Temporal ResolutionNormal Brain FunctioningNeurovascular DiseasesStrokeAlzheimer’s DiseaseHead InjuryOptical Imaging ModalitiesHemoglobin AbsorptionVisible And Near Infrared RangeMultispectral MeasurementBlood Oxygenation EstimationPartial Pressure Of Oxygen (pO2)Oxygen-sensitive Dyes
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Cite this Article
Sakadžić, S., Roussakis, E., Yaseen, M. A., Mandeville, E. T., Srinivasan, V. J., Arai, K., Ruvinskaya, S., Wu, W., Devor, A., Lo, E. H., Vinogradov, S. A., Boas, D. A. Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence. J. Vis. Exp. (51), e1694, doi:10.3791/1694 (2011).
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