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Cerebral Blutoxygenierung Messung auf Sauerstoff-abhängige Quenching der Phosphoreszenz Based
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Neuroscience
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JoVE Journal Neuroscience
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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Cerebral Blutoxygenierung Messung auf Sauerstoff-abhängige Quenching der Phosphoreszenz Based

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14,526 Views

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08:58 min

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May 04, 2011

DOI:

10.3791/1694-v

DOI:
10.3791/1694-v

•

08:58 min
May 04, 2011

•

3 Views
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1Optics Division, Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology,Massachusetts General Hospital and Harvard Medical School, 2Department of Biochemistry and Biophysics,University of Pennsylvania, 3Neuroprotection Research Laboratory, Departments of Radiology and Neurology,Massachusetts General Hospital and Harvard Medical School, 4Departments of Neurosciences and Radiology,University of California

Summary

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Wir präsentieren ein experimentelles Verfahren zur Messung der Sauerstoff-Partialdruck (pO2) in zerebralen Gefäßen auf Sauerstoff-abhängigen Löschung der Phosphoreszenz basiert. Tierische Vorbereitung und bildgebenden Verfahren wurden sowohl für großes Sichtfeld CCD-basierten Imaging von pO2 in Ratten und 2-Photonen-Anregung Bildgebung des pO2 bei Mäusen beschrieben.

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Sakadžić, S., Roussakis, E., Yaseen, M. A., Mandeville, E. T., Srinivasan, V. J., Arai, K., Ruvinskaya, S., Wu, W., Devor, A., Lo, E. H., Vinogradov, S. A., Boas, D. A. Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence. J. Vis. Exp. (51), e1694, doi:10.3791/1694 (2011).

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