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Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence

Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence

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08:58 min

May 04, 2011

DOI:

08:58 min
May 04, 2011

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Summary

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We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.

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