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Cerebral Blood Oxygenatie meting op basis van zuurstof-afhankelijke Afschrikken van Fosforescentie
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Neuroscience
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JoVE Journal Neuroscience
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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Cerebral Blood Oxygenatie meting op basis van zuurstof-afhankelijke Afschrikken van Fosforescentie

Nederlands

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14,526 Views

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08:58 min

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May 04, 2011

DOI:

10.3791/1694-v

DOI:
10.3791/1694-v

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08:58 min
May 04, 2011

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3 Views
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1Optics Division, Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology,Massachusetts General Hospital and Harvard Medical School, 2Department of Biochemistry and Biophysics,University of Pennsylvania, 3Neuroprotection Research Laboratory, Departments of Radiology and Neurology,Massachusetts General Hospital and Harvard Medical School, 4Departments of Neurosciences and Radiology,University of California

Summary

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We presenteren een experimentele procedure voor het meten van de partiële druk van zuurstof (pO2) in cerebrale vaatstelsel op basis van zuurstof-afhankelijke quenching van fosforescentie. Dier voorbereiding en beeldvormende procedures werden uiteengezet voor zowel grote beeldveld van CCD-gebaseerde beeldvorming van pO2 in ratten en twee-foton excitatie gebaseerde beeldvorming van de pO2 in muizen.

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Sakadžić, S., Roussakis, E., Yaseen, M. A., Mandeville, E. T., Srinivasan, V. J., Arai, K., Ruvinskaya, S., Wu, W., Devor, A., Lo, E. H., Vinogradov, S. A., Boas, D. A. Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence. J. Vis. Exp. (51), e1694, doi:10.3791/1694 (2011).

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