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Ossigenazione cerebrale misura Sangue Sulla base di ossigeno-dipendente tempra di fosforescenza
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Neuroscience
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JoVE Journal Neuroscience
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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Ossigenazione cerebrale misura Sangue Sulla base di ossigeno-dipendente tempra di fosforescenza

italiano

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14,526 Views

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08:58 min

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May 04, 2011

DOI:

10.3791/1694-v

DOI:
10.3791/1694-v

•

08:58 min
May 04, 2011

•

3 Views
, , , , , , , , , , ,
1Optics Division, Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology,Massachusetts General Hospital and Harvard Medical School, 2Department of Biochemistry and Biophysics,University of Pennsylvania, 3Neuroprotection Research Laboratory, Departments of Radiology and Neurology,Massachusetts General Hospital and Harvard Medical School, 4Departments of Neurosciences and Radiology,University of California

Summary

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Vi presentiamo una procedura sperimentale per la misurazione della pressione parziale di ossigeno (pO2) in vasi cerebrali sulla base di ossigeno-dipendente tempra di fosforescenza. Preparazione degli animali e delle procedure di imaging sono stati delineati per entrambi ampio campo visivo CCD-based imaging di pO2 nel ratto e nel 2-fotoni immagini eccitazione base di pO2 nei topi.

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Sakadžić, S., Roussakis, E., Yaseen, M. A., Mandeville, E. T., Srinivasan, V. J., Arai, K., Ruvinskaya, S., Wu, W., Devor, A., Lo, E. H., Vinogradov, S. A., Boas, D. A. Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence. J. Vis. Exp. (51), e1694, doi:10.3791/1694 (2011).

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