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Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription
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Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription

Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription

2,348 Views

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06:09 min

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August 11, 2021

DOI:

10.3791/62723-v

DOI:
10.3791/62723-v

•

06:09 min
August 11, 2021

•

7 Views
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1Department of Translational Medicine and Physiology, Elson S. Floyd College of Medicine,Washington State University

Summary

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Here, we describe a detailed protocol for isolating active nuclear extract from larval stage 4 C. elegans and visualizing transcription activity in an in vitro system.

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Caenorhabditis ElegansNuclear ExtractIn Vitro TranscriptionBalch HomogenizerHypotonic BufferHypertonic BufferSynchronizationL1 AnimalsL4 StageOP50CentrifugationHomogenizationTungsten Carbide Ball

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Wibisono, P., Sun, J. Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription. J. Vis. Exp. (174), e62723, doi:10.3791/62723 (2021).

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