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Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription
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Biochemie
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JoVE Journal Biochemie
Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription

Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription

2,348 Views

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06:09 min

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August 11, 2021

DOI:

10.3791/62723-v

DOI:
10.3791/62723-v

•

06:09 min
August 11, 2021

•

7 Views
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1Department of Translational Medicine and Physiology, Elson S. Floyd College of Medicine,Washington State University

Summary

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Here, we describe a detailed protocol for isolating active nuclear extract from larval stage 4 C. elegans and visualizing transcription activity in an in vitro system.

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Caenorhabditis ElegansNuclear ExtractIn Vitro TranscriptionBalch HomogenizerHypotonic BufferHypertonic BufferSynchronizationL1 AnimalsL4 StageOP50CentrifugationHomogenizationTungsten Carbide Ball

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Cite this Article

Wibisono, P., Sun, J. Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription. J. Vis. Exp. (174), e62723, doi:10.3791/62723 (2021).

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