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Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription
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Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription
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August 11, 2021
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Here, we describe a detailed protocol for isolating active nuclear extract from larval stage 4 C. elegans and visualizing transcription activity in an in vitro system.
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Wibisono, P., Sun, J. Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription. J. Vis. Exp. (174), e62723, doi:10.3791/62723 (2021).
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