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Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription

Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription

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06:09 min

August 11, 2021

DOI:

06:09 min
August 11, 2021

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Summary

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Here, we describe a detailed protocol for isolating active nuclear extract from larval stage 4 C. elegans and visualizing transcription activity in an in vitro system.

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