Here, we describe a detailed protocol for isolating active nuclear extract from larval stage 4 C. elegans and visualizing transcription activity in an in vitro system.
Wibisono, P., Sun, J. Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription. J. Vis. Exp. (174), e62723, doi:10.3791/62723 (2021).