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Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription
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JoVE Journal Biyokimya
Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription

Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription

2,345 Views

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06:09 min

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August 11, 2021

DOI:

10.3791/62723-v

DOI:
10.3791/62723-v

•

06:09 min
August 11, 2021

•

7 Views
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1Department of Translational Medicine and Physiology, Elson S. Floyd College of Medicine,Washington State University

Özet

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Here, we describe a detailed protocol for isolating active nuclear extract from larval stage 4 C. elegans and visualizing transcription activity in an in vitro system.

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Cite this Article

Wibisono, P., Sun, J. Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription. J. Vis. Exp. (174), e62723, doi:10.3791/62723 (2021).

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