JOVE JOURNAL

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Journal (Developmental Biology)

Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation

Karin Tourle, Amber Rucinski, Alexander Grainger, Ioannis J. Limnios, Macarena Gonzalez Ruiz, Jonathan K.H. Tan

¹Clem Jones Centre for Regenerative Medicine, Faculty of Health Sciences and Medicine,Bond University

Journal (Developmental Biology)

Methods to Enable Spatial Transcriptomics of Bone Tissues

Luigi Mancinelli², Karen Elizabeth Schoedel⁴, Kurt Richard Weiss^6,7, Giuseppe Intini^2,8,9,10

¹Department of Periodontics and Preventive Dentistry,University of Pittsburgh School of Dental Medicine, ²Center for Craniofacial Regeneration,University of Pittsburgh School of Dental Medicine, ³UPMC Presbyterian, ⁴UPMC Shadyside, ⁵Musculoskeletal Oncology Laboratory, Department of Orthopaedic Surgery,University of Pittsburgh, ⁶UPMC Hillman Cancer Center, ⁷Departments of Anatomic Pathology and General Surgical Oncology,University of Pittsburgh, ⁸Department of Medicine,University of Pittsburgh School of Medicine, ⁹University of Pittsburgh UPMC Hillman Cancer Center, ¹⁰McGowan Institute for Regenerative Medicine,University of Pittsburgh

Journal (Developmental Biology)

Isolation and Sectioning of Bone Tissue from Mouse for Transcriptomic Studies

Luigi Mancinelli², Karen Elizabeth Schoedel⁴, Kurt Richard Weiss^6,7, Giuseppe Intini^2,8,9,10

Journal (Developmental Biology)

Generation of Germ-Free Zebrafish Models from Embryos

Pan-Pan Jia, Xiang-Ru Liu, Ming-Fei Wu, Yan Li, Lan-Chen Zhang, De-Sheng Pei

¹School of Public Health,Chongqing Medical University

Journal (Developmental Biology)

GF Artemia nauplii Preparation for Chronic GF Zebrafish Nutrition

Pan-Pan Jia, Xiang-Ru Liu, Ming-Fei Wu, Yan Li, Lan-Chen Zhang, De-Sheng Pei

¹School of Public Health,Chongqing Medical University

Journal (Developmental Biology)

Tracking Germ-Free Zebrafish Models Across All Life Stages

Pan-Pan Jia, Xiang-Ru Liu, Ming-Fei Wu, Yan Li, Lan-Chen Zhang, De-Sheng Pei

¹School of Public Health,Chongqing Medical University

Journal (Developmental Biology)

Generation, Maintenance, and Identification of Germ-Free Zebrafish Models from Larvae to Juvenile Stages

Pan-Pan Jia, Xiang-Ru Liu, Ming-Fei Wu, Yan Li, Lan-Chen Zhang, De-Sheng Pei

¹School of Public Health,Chongqing Medical University

Journal (Developmental Biology)

Generation and Downstream Analysis of Single-Cell and Single-Nuclei Transcriptomes in Brain Organoids

Miriam Wandres*^1,2, Denise Aigner*^1,2, Nicolai Kastelic³, Anastasiya Boltengagen, Agnieszka Rybak-Wolf³, Nikolaus Rajewsky²

¹Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine (MDC), ²Institut für Biologie,Humboldt-Universität zu Berlin, ³Organoid platform, Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine (MDC)

Journal (Developmental Biology)

Isolation of Single Cells from Human Brain Organoids for Single-Cell RNA Sequencing

Miriam Wandres*^1,2, Denise Aigner*^1,2, Nicolai Kastelic³, Anastasiya Boltengagen, Agnieszka Rybak-Wolf³, Nikolaus Rajewsky²

Journal (Developmental Biology)

Isolation of Single Nuclei from Human Brain Organoids for Single-Nucleus RNA Sequencing

Miriam Wandres*^1,2, Denise Aigner*^1,2, Nicolai Kastelic³, Anastasiya Boltengagen, Agnieszka Rybak-Wolf³, Nikolaus Rajewsky²

Journal (Developmental Biology)

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Alexander Blümke², Jessica Simon, Elizabeth Leber, Marta Scatena, Cecilia M. Giachelli

¹Department of Bioengineering, Department of Medicine,University of Washington, ²Department of Orthopedics and Trauma Surgery, Medical Faculty Mannheim,Heidelberg University

Journal (Developmental Biology)

Nuclear Isolation from Cryopreserved In Vitro Derived Blood Cells

Rong Qiu, Chayanne Petit, Christopher S. Thom²

¹Division of Neonatology,Children’s Hospital of Philadelphia, ²Department of Pediatrics,University of Pennsylvania Perelman School of Medicine

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