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细菌中的RIBO-seq：NGS测序样本收集和库准备协议

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RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

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细菌中的RIBO-seq：NGS测序样本收集和库准备协议

DOI:

10.3791/62544-v

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12:05 min

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August 07, 2021

•

Natalia Kopik², Olga Chrobak², Przemyslaw Latoch³, Mariia Kovalenko², Agata L. Starosta²

¹Laboratory of Gene Expression, ECOTECH-Complex,Maria Curie-Sklodowska University, ²Department of Molecular Biology, Institute of Biological Sciences,Maria Curie-Sklodowska University, ³Faculty of Information Technology,Polish-Japanese Academy of Information Technology

Chapters

00:00Introduciton
00:54Cell Harvesting and Lysis
04:13MNase Digestion and Size Selection of Samples for RIBO-seq
06:17rRNA Depletion and Alkaline Fragmentation of Samples for RNA-seq
07:07Library Preparation and Size Selection of cDNA Libraries
08:49Results: Quality of cDNA Libraries and Reads
11:31Conclusion

Summary

자동 번역

在这里，我们描述了细菌中RIBO-seq样品收集和制备的阶段。根据这些准则对图书馆进行排序，可以获得足够的数据，以便进行全面的生物信息分析。我们目前的协议很简单，使用标准的实验室设备，从裂解到获得库需要七天时间。

Tags

RIBO-seq Ribosome Profiling Protein Synthesis Translation RNA Sequencing Bacterial Culture Sample Collection Library Preparation NGS Sequencing

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