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RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
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RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
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August 07, 2021
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Summary
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Here we describe the stages of sample collection and preparation for RIBO-seq in bacteria. Sequencing of the libraries prepared according to these guidelines results in sufficient data for comprehensive bioinformatic analysis. The protocol we present is simple, uses standard laboratory equipment and takes seven days from lysis to obtaining libraries.
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Kopik, N., Chrobak, O., Latoch, P., Kovalenko, M., Starosta, A. L. RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing. J. Vis. Exp. (174), e62544, doi:10.3791/62544 (2021).
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