/
/
RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
7,500 Views
•
12:05 min
•
August 07, 2021
DOI:
Summary
Automatically generated
Here we describe the stages of sample collection and preparation for RIBO-seq in bacteria. Sequencing of the libraries prepared according to these guidelines results in sufficient data for comprehensive bioinformatic analysis. The protocol we present is simple, uses standard laboratory equipment and takes seven days from lysis to obtaining libraries.
Read Article
Cite this Article
Kopik, N., Chrobak, O., Latoch, P., Kovalenko, M., Starosta, A. L. RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing. J. Vis. Exp. (174), e62544, doi:10.3791/62544 (2021).
Copy