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Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
Chapters
- 00:04Title
- 00:52OpenProt Database Download
- 01:53Database Handling
- 02:41Mass Spectrometry File Preparation
- 03:13Protein Quantification
- 04:13Quality Control and OpenProt Database Mining
- 05:25Results: Representative Interactor and Mass Spectrometry Identification of a Novel Protein
- 06:40Conclusion
OpenProt is a freely accessible database that enforces a polycistronic model of eukaryotic genomes. Here, we present a protocol for the use of OpenProt databases when interrogating mass spectrometry datasets. Using OpenProt database for analysis of proteomic experiments allows for discovery of novel and previously undetectable proteins.
