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Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
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Genética
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JoVE Journal Genética
Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

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07:38 min

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April 11, 2019

DOI:

10.3791/59589-v

DOI:
10.3791/59589-v

•

07:38 min
April 11, 2019

•

4 Views
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1Department of Biochemistry,Université de Sherbrooke, 2PROTEO,Quebec Network for Research on Protein Function, Structure, and Engineering

Summary

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OpenProt is a freely accessible database that enforces a polycistronic model of eukaryotic genomes. Here, we present a protocol for the use of OpenProt databases when interrogating mass spectrometry datasets. Using OpenProt database for analysis of proteomic experiments allows for discovery of novel and previously undetectable proteins.

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Mass SpectrometryProteomicsOpenProt DatabaseNon-canonical Open Reading FramesNovel ProteinsProteomeProteomic AnalysesDatabase HandlingMS Convert ToolMzML File Format

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Brunet, M. A., Roucou, X. Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames. J. Vis. Exp. (146), e59589, doi:10.3791/59589 (2019).

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