Journal
/
발생학
/
An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

10,623 Views

•

05:48 min

•

December 02, 2018

DOI:

10.3791/58512-v

DOI:
10.3791/58512-v

•

05:48 min
December 02, 2018

•

5 Views
, , , , , ,
1State Key Laboratory of Reproductive Medicine,Nanjing Medical University, 2Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine,University of Science and Technology of China

Summary

Automatically generated

Here, we present a protocol to assess the blood-testis barrier integrity by injecting inulin-FITC into testes. This is an efficient in vivo method to study blood-testis barrier integrity that can be compromised by genetic and environmental elements.

Explore More Videos

Blood-testis BarrierIn Vivo MethodMouseCadmium ChlorideTestisInulin-FITCMicroinjectionCryosectioningParaformaldehydeOptimal Cutting Temperature Compound

Read Article

Cite this Article

Liu, M., Zhu, C., Bai, S., Li, X., Fu, K., Ye, L., Zheng, K. An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity. J. Vis. Exp. (142), e58512, doi:10.3791/58512 (2018).

Download .ris file

Copy

Share Video

.

Copy