Journal
/
Biología del desarrollo
/
An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

DOI:
10.3791/58512-v

•

05:48 min

•

December 02, 2018

•

, , , , , ,
1State Key Laboratory of Reproductive Medicine,Nanjing Medical University, 2Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine,University of Science and Technology of China

Capítulos

  • 00:04Título
  • 00:25Inulin-FITC Administration
  • 02:20Tissue Harvest and Imaging
  • 04:00Results: Representative Blood-Testis Barrier (BTB) Integrity Assessment
  • 04:50Conclusion

Summary

Traducción Automática

Traducción Automática

Here, we present a protocol to assess the blood-testis barrier integrity by injecting inulin-FITC into testes. This is an efficient in vivo method to study blood-testis barrier integrity that can be compromised by genetic and environmental elements.

Tags

Blood-testis BarrierIn Vivo MethodMouseCadmium ChlorideTestisInulin-FITCMicroinjectionCryosectioningParaformaldehydeOptimal Cutting Temperature Compound

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

An Efficient Method to Obtain Dedifferentiated Fat Cells

In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition

An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks

Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo

Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues

A Hyperandrogenic Mouse Model to Study Polycystic Ovary Syndrome

Read Article