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An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

10,619 Views

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05:48 min

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December 02, 2018

DOI:

10.3791/58512-v

DOI:
10.3791/58512-v

•

05:48 min
December 02, 2018

•

5 Views
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1State Key Laboratory of Reproductive Medicine,Nanjing Medical University, 2Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine,University of Science and Technology of China

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Here, we present a protocol to assess the blood-testis barrier integrity by injecting inulin-FITC into testes. This is an efficient in vivo method to study blood-testis barrier integrity that can be compromised by genetic and environmental elements.

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Liu, M., Zhu, C., Bai, S., Li, X., Fu, K., Ye, L., Zheng, K. An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity. J. Vis. Exp. (142), e58512, doi:10.3791/58512 (2018).

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