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An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

10,623 Views

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05:48 min

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December 02, 2018

DOI:

10.3791/58512-v

DOI:
10.3791/58512-v

•

05:48 min
December 02, 2018

•

5 Views
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1State Key Laboratory of Reproductive Medicine,Nanjing Medical University, 2Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine,University of Science and Technology of China

Summary

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Here, we present a protocol to assess the blood-testis barrier integrity by injecting inulin-FITC into testes. This is an efficient in vivo method to study blood-testis barrier integrity that can be compromised by genetic and environmental elements.

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Cite this Article

Liu, M., Zhu, C., Bai, S., Li, X., Fu, K., Ye, L., Zheng, K. An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity. J. Vis. Exp. (142), e58512, doi:10.3791/58512 (2018).

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