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An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity
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JoVE 杂志 发育生物学
An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

DOI:
10.3791/58512-v

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05:48 min

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December 02, 2018

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, , , , , ,
1State Key Laboratory of Reproductive Medicine,Nanjing Medical University, 2Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine,University of Science and Technology of China

Chapters

  • 00:04Title
  • 00:25Inulin-FITC Administration
  • 02:20Tissue Harvest and Imaging
  • 04:00Results: Representative Blood-Testis Barrier (BTB) Integrity Assessment
  • 04:50Conclusion

Summary

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Here, we present a protocol to assess the blood-testis barrier integrity by injecting inulin-FITC into testes. This is an efficient in vivo method to study blood-testis barrier integrity that can be compromised by genetic and environmental elements.

Tags

Blood-testis BarrierIn Vivo MethodMouseCadmium ChlorideTestisInulin-FITCMicroinjectionCryosectioningParaformaldehydeOptimal Cutting Temperature Compound

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