Journal
/
유전학
/
Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
JoVE 신문
유전학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 유전학
Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

DOI:
10.3791/57920-v

•

08:22 min

•

September 15, 2018

•

, ,
1Department of Cell and Developmental Biology,University College London, 2Yale Cardiovascular Research Group, Section of Cardiovascular Medicine,Yale School of Medicine, 3Yale Cardiovascular Research Center, Section of Cardiovascular Medicine,Yale School of Medicine

Chapters

  • 00:04Title
  • 00:24Tissue Preparation
  • 02:07Probe Hybridization
  • 03:55DIG Antibody Detection
  • 05:20cTNT Antibody Detection and DAPI Staining
  • 06:25Results: PIGF-overexpressing Heart Tissue and miRNA-182 Expression
  • 07:33Conclusion

Summary

자동 번역

자동 번역

micro-RNAs (miRNAs) are short and highly homologous RNA sequences, serving as post-transcriptional regulators of messenger RNAs (mRNAs). Current miRNA detection methods vary in sensitivity and specificity. We describe a protocol that combines in situ hybridization and immunostaining for concurrent detection of miRNA and protein molecules on mouse heart tissue sections.

Tags

MiRNAIn Situ HybridizationProtein DetectionRehydrationProteinase KFixationHybridizationProbeOptimization

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Read Article