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Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
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JoVE Journal 遺伝学
Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

DOI:
10.3791/57920-v

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08:22 min

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September 15, 2018

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, ,
1Department of Cell and Developmental Biology,University College London, 2Yale Cardiovascular Research Group, Section of Cardiovascular Medicine,Yale School of Medicine, 3Yale Cardiovascular Research Center, Section of Cardiovascular Medicine,Yale School of Medicine

章

  • 00:04標題
  • 00:24Tissue Preparation
  • 02:07Probe Hybridization
  • 03:55DIG Antibody Detection
  • 05:20cTNT Antibody Detection and DAPI Staining
  • 06:25Results: PIGF-overexpressing Heart Tissue and miRNA-182 Expression
  • 07:33Conclusion

概要

自動翻訳

自動翻訳

micro-RNAs (miRNAs) are short and highly homologous RNA sequences, serving as post-transcriptional regulators of messenger RNAs (mRNAs). Current miRNA detection methods vary in sensitivity and specificity. We describe a protocol that combines in situ hybridization and immunostaining for concurrent detection of miRNA and protein molecules on mouse heart tissue sections.

タグ

MiRNAIn Situ HybridizationProtein DetectionRehydrationProteinase KFixationHybridizationProbeOptimization

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