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Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
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Genética
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JoVE Journal Genética
Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

8,000 Views

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08:22 min

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September 15, 2018

DOI:

10.3791/57920-v

DOI:
10.3791/57920-v

•

08:22 min
September 15, 2018

•

3 Views
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1Department of Cell and Developmental Biology,University College London, 2Yale Cardiovascular Research Group, Section of Cardiovascular Medicine,Yale School of Medicine, 3Yale Cardiovascular Research Center, Section of Cardiovascular Medicine,Yale School of Medicine

Summary

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micro-RNAs (miRNAs) are short and highly homologous RNA sequences, serving as post-transcriptional regulators of messenger RNAs (mRNAs). Current miRNA detection methods vary in sensitivity and specificity. We describe a protocol that combines in situ hybridization and immunostaining for concurrent detection of miRNA and protein molecules on mouse heart tissue sections.

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Cite this Article

Memi, F., Tirziu, D., Papangeli, I. Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization. J. Vis. Exp. (139), e57920, doi:10.3791/57920 (2018).

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